|
KMID : 1094020170340030172
|
|
Journal of Veterinary Clinics
2017 Volume.34 No. 3 p.172 ~ p.177
|
|
|
Fucoidan Suppresses Prostaglandin E2 Production and Akt Activation in Lipopolysaccharide-Stimulated Porcine Peripheral Blood Mononuclear Cells
|
|
Park Geon-Tae
Ahn Chang-Hwan
Kang Byeong-Teck
Kang Ji-Houn
Jeung Eui-Bae
Yang Mhan-Pyo
|
|
|
Abstract
|
|
|
|
Fucoidan, a cell wall polysaccharide found in the brown seaweed, is reported to have broad-spectrum biological activities. The objectives of this study were to examine the effect of fucoidan on prostaglandin E2 (PGE2) and cyclooxygenase-2 (COX-2) expression in lipopolysaccharide (LPS)-stimulated porcine peripheral blood mononuclear cells (PBMCs) and to determine whether these effects are involved in Akt activation. The levels of PGE2 production in the culture supernatants from PBMCs were determined by the enzyme-linked immunosorbent assay (ELISA) kit and the levels of COX-2 mRNA were measured by real time polymerase chain reaction (RT-PCR). Akt activity was determined by Western blot analysis. Fucoidan in LPS-naive PBMCs has no effect on PGE2 production and COX-2 mRNA expression. Furthermore, fucoidan does not affect Akt activation in LPS- naive PBMCs. However, PGE2 production and COX-2 mRNA expression on PBMCs were remarkably enhanced by LPS stimulation. Akt activity was also increased by LPS. Increasing effects of PGE2 production and COX-2 mRNA expression in PBMCs induced by LPS were suppressed by addition of fucoidan. In addition, fucoidan reduced an increase in Akt activity in LPSstimulated PBMCs. These results suggested that fucoidan exerts potent anti-inflammatory properties by suppression of PGE2 production, COX-2 mRNA expression and Akt activation in LPS-stimulated PBMCs.
|
|
|
KEYWORD
|
|
|
fucoidan , Akt , anti-inflammation , PBMCs , PGE2 , COX-2 , porcine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|